Antimicrobial Effects of Annona squamosa Leaf Extract on Staphylococcus aureus Growth: An In Vitro Study

https://doi.org/10.33860/jbc.v5i2.2167

Authors

  • Dwi Dianita Irawan Department of Midwifery, Universitas Muhammadiyah Lamongan, Lamongan, Indonesia
  • Amrina Nur Rohmah Department of Midwifery, Universitas Muhammadiyah Lamongan, Lamongan, Indonesia
  • Elia Ika Rahmawati Department of Midwifery, Universitas Muhammadiyah Lamongan, Lamongan, Indonesia
  • Risya Secha Primindari Department of Midwifery, Universitas Muhammadiyah Lamongan, Lamongan, Indonesia
  • Shinta Alifiana Rahmawati Department of Midwifery, Universitas Muhammadiyah Lamongan, Lamongan, Indonesia

Keywords:

Staphylococcus aureus, Annona squamosa, Microbe, Bacteria, in vitro

Abstract

Introduction: Mastitis is inflammation of the breast tissue due to infection with the Staphylococcus aureus bacteria causing local infections. It takes the natural ingredient Annona squamosal extract as an antimicrobial. Purpose: to determine the effectiveness of Annona squamosa leaf extract on the inhibition and killing power of Staphylococcus. Method: Experimental laboratory research with antibacterial activity test. Tube dilution method by incorporating bacteria and extracts with concentrations of 20%, 22.5%, 25%, 27.5%, 30%, and 32.5% and then measuring the MIC. The diffusion method is carried out by planting bacteria in a Nutrient Agar Plate and then giving the extract with 6 concentrations to calculate the MBC. Results: The results of the One-Way ANOVA test obtained a significance value of (p<0.001), indicating that changes in the concentration of Annona squamosa made a significant difference to Staphylococcus. The correlation test showed a significant p-value of (p<0.001), which means that there was a significant relationship between the administration of extract and the number of Staphylococcus. Conclusion: Annona squamosa leaf extract is able to significantly inhibit the growth of Staphylococcus bacteria and the higher the concentration of Annona squamosa leaf extract, the fewer the number of bacterial colonies that grow.

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Published

2023-06-30

How to Cite

Irawan, D. D., Rohmah, A. N., Rahmawati, E. I., Primindari, R. S. ., & Rahmawati, S. A. (2023). Antimicrobial Effects of Annona squamosa Leaf Extract on Staphylococcus aureus Growth: An In Vitro Study. Jurnal Bidan Cerdas, 5(2), 58–65. https://doi.org/10.33860/jbc.v5i2.2167

Issue

Vol. 5 No. 2 (2023)

Section

Articles
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Copyright (c) 2023 Dwi Dianita Irawan, Amrina Nur Rohmah, Elia Ika Rahmawati, Risya Secha Primindari, Shinta Alifiana Rahmawati

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