Detection of Dengue Virus in Female Aedes aegypti Mosquito using Reverse Transcription-polymerase Chain Reaction (RT-PCR) in West Jakarta


  • Dessy Paiman Department of Environmental Health, Diponegoro University, Semarang, Central Java, Indonesia
  • Ony Setiani Department of Environmental Health, Diponegoro University, Semarang, Central Java, Indonesia
  • Budiyono Budiyono Department of Environmental Health, Diponegoro University, Semarang, Central Java, Indonesia


Aedes aegypti, Dengue, RT-PCR


Dengue hemorrhagic fever (DHF) is an acute disease caused by dengue virus infection which is a clinical manifestation of bleeding, transmitted through the bite of a female mosquito Aedes aegypti. The incidence of dengue fever is still a health problem in developing countries, including Indonesia. There are several ways to detect the presence of dengue virus, namely by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The purpose of this study was to detect Dengue virus in adult female Aedes aegypti mosquitoes using RT-PCR. The type of research is a descriptive survey to determine the number of dengue virus transmission in Aedes aegypti mosquitoes in Kembangan Village, West Jakarta, especially in RW02 RT 1-10. There are 62 Aedes aegypties found in 100 houses that were selected by simple random sampling. Totally 23 female Aedes aegypties were identified from a total of 62. Moreover, the result showed that all Aedes aegypti mosquitoes caught in were negative for dengue virus. There are several reasons for the limitation of RT-PCR that caused zero findings, including potential false negatives, sensitivity, low quality control measures. Moreover, the small sample size and seasonal reason also played a role in impacting the zero result.


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How to Cite

Paiman, D., Setiani, O., & Budiyono, B. (2024). Detection of Dengue Virus in Female Aedes aegypti Mosquito using Reverse Transcription-polymerase Chain Reaction (RT-PCR) in West Jakarta. Poltekita : Jurnal Ilmu Kesehatan, 17(4), 1250–1256.



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