Effect of Lycopene Administration on the Antioxidant Status of Hypercholesterolemic Wistar Rats (Rattus Norvegicus)



  • Vina Ariesta Dewi Department of Biomedicine, University of Prima Indonesia, Medan, Indonesia
  • Silvia Fransisca Department of Biomedicine, University of Prima Indonesia, Medan, Indonesia
  • Wiiliam Leslie Department of Biomedicine, University of Prima Indonesia, Medan, Indonesia
  • Rico Alexander Department of Biomedicine, University of Prima Indonesia, Medan, Indonesia


Lycopene, Free Radicals, Antioxidant


An imbalance between oxidants and antioxidants causes oxidative stress. Antioxidants such as vitamins C and E, known to prevent various diseases, can help reduce oxidative stress. The antioxidant lycopene in fruits can prevent carcinogenesis and atherogenesis. The objective of this study is to assess the impact of administering lycopene at doses of 0.36, 0.72, and 1.08 mg/day from tomato (Lycopersicum esculentum) fruit extract on the antioxidant levels (Vitamin C, E, and GPx) in hypercholesterolemic Wistar rats (Rattus norvegicus). The research methods of this study employ true experiment designs, LSD post hoc, control, and treatment groups. Experimentally, 28 rats with the Wistar strain were assigned to control and treatment groups. Hypercholesterolemic rats were fed high-fat, high-cholesterol with 0.35 ml/day cholesterol crystals. Vitamin C, vitamin E, and GPx levels were measured. The result of this study shows lycopene increased both vitamin C and E (P3 > P2 > P1 > P0). A post hoc LSDV statistical test of vitamin C, vitamin E, and GPX levels shows a significant difference (p = 0.00). The conclusion of this study found that administering a dosage of 0.36 mg/head/day of lycopene to those with high cholesterol levels benefits the body's antioxidant status, thereby improving the overall ability to counteract oxidative stress.


Jousilahti P, Laatikainen T, Salomaa V, Pietilä A, Vartiainen E, Puska P. 40-Year CHD Mortality Trends and the Role of Risk Factors in Mortality Decline: The North Karelia Project Experience. Glob Heart. 2016 Jun 1;11(2):207–12.

Rodgers JL, Jones J, Bolleddu SI, Vanthenapalli S, Rodgers LE, Shah K, et al. Cardiovascular Risks Associated with Gender and Aging. Journal of Cardiovascular Development and Disease 2019, Vol 6, Page 19 [Internet]. 2019 Apr 27 [cited 2024 Feb 3];6(2):19. Available from: https://www.mdpi.com/2308-3425/6/2/19/htm

Zhang Q, Ai Y, Dong H, Wang J, Xu L. Circulating Oxidized Low-Density Lipoprotein is a Strong Risk Factor for the Early Stage of Coronary Heart Disease. IUBMB Life [Internet]. 2019 Feb 1 [cited 2024 Feb 2];71(2):277–82. Available from: https://onlinelibrary.wiley.com/doi/full/10.1002/iub.1966

Bhattacharya S. Reactive oxygen species and cellular defense system. Free Radicals in Human Health and Disease [Internet]. 2015 Jan 1 [cited 2024 Jan 30];17–29. Available from: https://link.springer.com/chapter/10.1007/978-81-322-2035-0_2

Voronkova EANI; S, Voronkova YS, Gorban OS, Holoborodko VA. Oxidative stress, reactive oxygen species, antioxidants: a review. Ecology and Noospherology [Internet]. 2018 May 9 [cited 2024 Feb 3];29(1):52–5. Available from: https://en.dp.ua/index.php/en/article/view/118

Tauffenberger A, Magistretti PJ. Reactive Oxygen Species: Beyond Their Reactive Behavior. Neurochem Res. 2021 Jan 13;46(1):77–87.

Checa J, Aran JM. Reactive oxygen species: Drivers of physiological and pathological processes. J Inflamm Res. 2020;13:1057–73.

Nakamura H, Takada K. Reactive oxygen species in cancer: Current findings and future directions. Cancer Sci [Internet]. 2021 Oct 1 [cited 2024 Feb 8];112(10):3945–52. Available from: https://onlinelibrary.wiley.com/doi/full/10.1111/cas.15068

Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Archives of Toxicology 2023 97:10 [Internet]. 2023 Aug 19 [cited 2024 Feb 8];97(10):2499–574. Available from: https://link.springer.com/article/10.1007/s00204-023-03562-9

Marreiro D do N, Cruz KJC, Morais JBS, Beserra JB, Severo JS, Soares de Oliveira AR. Zinc and Oxidative Stress: Current Mechanisms. Antioxidants (Basel) [Internet]. 2017 Jun 1 [cited 2024 Jan 27];6(2). Available from: https://pubmed.ncbi.nlm.nih.gov/28353636/

Recknagel RO, Glende EA, Britton RS. Free Radical Damage and Lipid Peroxidation. Hepatotoxicology [Internet]. 2020 Jan 16 [cited 2024 Feb 3];401–36. Available from: https://www.taylorfrancis.com/chapters/edit/10.1201/9780367812041-9/free-radical-damage-lipid-peroxidation-richard-recknagel-eric-glende-robert-britton

Nakai K, Tsuruta D. What Are Reactive Oxygen Species, Free Radicals, and Oxidative Stress in Skin Diseases? International Journal of Molecular Sciences 2021, Vol 22, Page 10799 [Internet]. 2021 Oct 6 [cited 2024 Feb 8];22(19):10799. Available from: https://www.mdpi.com/1422-0067/22/19/10799/htm

Engwa GA. Free radicals and the role of plant phytochemicals as antioxidants against oxidative stress-related diseases. Phytochemicals: source of antioxidants and role in disease prevention BoD–Books on Demand. 2018;7:49–74.

Duke JA, Bogenschutz-Godwin MJ, DuCellier J, Duke PAK, Kumar R. Handbook of Medicinal Herbs Second Edition (Kindle Edi, Vol. 5, Issue 1). Florida: CRC Press. 2022.

Yaman SO, Ayhanci A, Yaman SO, Ayhanci A. Lipid Peroxidation. Eur J Clin Nutr [Internet]. 2021 Feb 12 [cited 2024 Jan 27];47(11):759–64. Available from: https://www.intechopen.com/chapters/75229

Chaudhari PM, Paithankar A V. Herbal Nanogel Formulation: A Novel Approch. Journal of Science and Technology [Internet]. [cited 2024 Feb 8];5(5):149–53. Available from: www.jst.org.inDOI:https://doi.org/10.46243/jst.2020.v5.i5.pp149-153

Qazi MA, Molvi KI. Free Radicals and their Management. Am J Pharm Health Res [Internet]. 2018;6(04). Available from: www.ajphr.com

Mirahmadi M, Azimi-Hashemi S, Saburi E, Kamali H, Pishbin M, Hadizadeh F. Potential inhibitory effect of lycopene on prostate cancer. Biomedicine & Pharmacotherapy. 2020 Sep 1;129:110459.

Liu R, Mabury SA. Synthetic Phenolic Antioxidants: A Review of Environmental Occurrence, Fate, Human Exposure, and Toxicity. Environ Sci Technol [Internet]. 2020 Oct 6 [cited 2024 Feb 3];54(19):11706–19. Available from: https://pubs.acs.org/doi/abs/10.1021/acs.est.0c05077

Moradi B, Abbaszadeh S, Shahsavari S, Alizadeh M, Beyranvand F. The most useful medicinal herbs to treat diabetes. Biomedical Research and Therapy. 2018;5(8):2538–51.

Notoatmodjo S. Metodologi Penelitian Kesehatan. 3rd ed. Jakarta: Rineka Cipta; 2022. 268 p.

Weichbrod RH, Thompson GA (Heidbrink), Norton JN. Management of Animal Care and Use Programs in Research, Education, and Testing. 2nd ed. Boca Raton: CRC Press Taylor & Francis Group; 2018. 902 p.

Ghozali I. Aplikasi Analisis Multivariate dengan Program IBM SPSS 25. Badan Penerbit Universitas Diponegoro. Semarang; 2018.

Federer WT. Randomization and Sample Size In Experimentation. Cornell University Biometrics Unit Technical [Internet]. 1966 Sep [cited 2024 Feb 7];Number BU-236-M:1–15. Available from: https://hdl.handle.net/1813/32334

Suwarno B, Nugroho A. Kumpulan Variabel-Variabel Penelitian Manajemen Pemasaran (Definisi & Artikel Publikasi). 1st ed. Bogor: Halaman Moeka Publishing; 2023. 207 p.

Ligor M, Buszewski B. Thin Layer Chromatographic Techniques (TLC, OP TLC) for Determination of Biological Activated Compounds from Herb Extracts. J Liq Chromatogr Relat Technol [Internet]. 2007 Jan [cited 2024 Feb 6];30(17):2617–28. Available from: https://www.tandfonline.com/doi/abs/10.1080/10826070701540639

Šegan S, Opsenica D, Milojković-Opsenica D. Thin-layer chromatography in medicinal chemistry. J Liq Chromatogr Relat Technol [Internet]. 2019 Jun 15 [cited 2024 Feb 3];42(9–10):238–48. Available from: https://www.tandfonline.com/doi/abs/10.1080/10826076.2019.1585615

Rosnah R, Taslim NA, Aman AM, Idris I, As’ad S, Buchari A, et al. The Formulation and Evaluation of High-Fat Pellet on Lipid Profiles and Body Mass Index of Male Wistar Rats. Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) [Internet]. 2022 Jun 23 [cited 2024 Feb 8];31(1):285–92. Available from: https://www.bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1555

Arballo J, Amengual J, Erdman JW. Lycopene: A Critical Review of Digestion, Absorption, Metabolism, and Excretion. Antioxidants 2021, Vol 10, Page 342 [Internet]. 2021 Feb 25 [cited 2024 Feb 8];10(3):342. Available from: https://www.mdpi.com/2076-3921/10/3/342/htm

Narasimhulu CA, Parthasarathy S. Preparation of LDL, Oxidation, Methods of Detection, and Applications in Atherosclerosis Research. Methods Protoc. 2022;213.

Przybylska S. Lycopene – a bioactive carotenoid offering multiple health benefits: a review. Int J Food Sci Technol [Internet]. 2020 Jan 1 [cited 2024 Feb 3];55(1):11–32. Available from: https://onlinelibrary.wiley.com/doi/full/10.1111/ijfs.14260

Szarka A, Kapuy O, Lorincz T, Bánhegyi G. Vitamin C and Cell Death. Antioxid Redox Signal [Internet]. 2021 Apr 10 [cited 2024 Feb 8];34(11):831–44. Available from: https://www.liebertpub.com/doi/10.1089/ars.2019.7897

Bayram I, Decker EA. Underlying mechanisms of synergistic antioxidant interactions during lipid oxidation. Trends Food Sci Technol. 2023 Mar 1;133:219–30.

Averill-Bates DA. The antioxidant glutathione. Vitam Horm. 2023 Jan 1;121:109–41.



How to Cite

Dewi, V. A., Fransisca, S., Leslie, W., & Alexander, R. (2024). Effect of Lycopene Administration on the Antioxidant Status of Hypercholesterolemic Wistar Rats (Rattus Norvegicus). Poltekita : Jurnal Ilmu Kesehatan, 17(4), 1263–1269. https://doi.org/10.33860/jik.v17i4.3572



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