The Effect of Extra Virgin Olive Oil on Reducing Malondialdehyde Levels and Liver Histopathology in Male Rattus Norvegicus with Hypercholesterolemia
Keywords:
EVOO, MDA Levels, Hypercholesterolemia, LiverAbstract
All living things age physiologically. Free radicals attack cells as they age, causing damage, disruption, and death. Free radicals oxidize and damage cell membrane lipids. The body needs optimum antioxidants during oxidative stress. This study hypothesizes EVOO can lower MDA and total cholesterol and repair liver histopathology in hypercholesterolemic Wistar male rats—this research with a pre-post-test control group experiment. The study found that the control group's MDA level was 0.14 ± 0.03 nmol/ml before treatment (pretest), while the group given standard meal for mice and distilled water/day/head for 14 days had a drop to 0.13 ± 0.10 nmol/ml. In treatment group 1, the average MDA level was 0.73 ± 0.03 nmol/ml before treatment, and after 14 days of high-fat, high-cholesterol meal without EVOO, the average MDA level was 0.73 ± 0.03 nmol/ml and dropped to 0.48 ± 0.10 nmol/ml. Treatment group 2 had an average MDA level of 0.22 ± 0.02 nmol/ml before treatment, but after receiving a high-fat, high-cholesterol diet and a 1cc dose of EVOO for 14 days, it decreased to 0.17 ± 0.02 nmol/ml. Before therapy, the mean MDA level in group 3 was 0.17 ± 0.02 nmol/mL. Giving mice a lot of fat and cholesterol and a two cc dose of EVOO for 14 days lowered their MDA levels to 0.08 ± 0.01 nmol/ml, ranging from 0.10 to 0.05. The research concluded that average MDA decreased significantly in treatment group 3 after mice were fed high-fat, high-cholesterol feed and given two ccs of EVOO.
References
Jiang H, Zhou Y, Nabavi SM, Sahebkar A, Little PJ, Xu S, et al. Mechanisms of Oxidized LDL-Mediated Endothelial Dysfunction and Its Consequences for the Development of Atherosclerosis. Front Cardiovasc Med. 2022;9(June):1–11.
Ryu BH. Low Density Lipoprotein(LDL), Atherosclerosis and Antioxidants. Biotechnology and Bioprocess Engineering. 2000;5:313–9.
Poznyak A V., Grechko A V., Orekhova VA, Chegodaev YS, Wu WK, Orekhov AN. Oxidative Stress and Antioxidants in Atherosclerosis Development and Treatment. Biology (Basel). 2020;9(3):60–74.
Hemmati AA, Aghel N, Rashidi I, Gholampur-Aghdami A. Topical grape (Vitis vinifera) seed extract promotes repair of full thickness wound in rabbit. Int Wound J. 2011;8(5):514–20.
PERKI. Panduan Tata Laksana Dislipidemia 2022 [Internet]. 2020th ed. Erwinanto, Ng S, Santoso A, Desandri DR, Erika, Sukmawan R, et al., editors. Jakarta: Perhimpunan Dokter Spesialis Kardiovaskular Indonesia Panduan; 2022. 110 p. Available from: http://inaheart.org/panduan-tatalaksana-dislipidemia-2022/
Huff T, Boyd B, Jialal I. Physiology, Cholesterol [Internet]. Creighton University School of Medicine: StatPearls Publishing, Treasure Island (FL); 2023. Available from: http://europepmc.org/abstract/MED/29262185
Wang HH, Garruti G, Liu M, Portincasa P, Wang DQH. Cholesterol and lipoprotein metabolism and atherosclerosis: Recent advances in reverse cholesterol transport. Ann Hepatol. 2017;16:s27–42.
Amable PR, Carias RBV, Teixeira MVT, Da Cruz Pacheco Í, Corrêa Do Amaral RJF, Granjeiro JM, et al. Platelet-rich plasma preparation for regenerative medicine: Optimization and quantification of cytokines and growth factors. Stem Cell Res Ther. 2013;4(3):1–13.
Susantiningsih T, Mustofa S. Ekspresi IL-6 dan TNF-α Pada Obesitas. Jurnal Kedokteran Universitas Lampungila. 2018;2(2):174–80.
Yaman SO, Ayhanci A. Lipid Peroxidation. In: Atukeren P, editor. Accenting Lipid Peroxidation [Internet]. London: Intech Open; 2021. p. 1–13. Available from: https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics
Rastegar Moghaddam Mansouri M, Abbasian S, Khazaie M. Melatonin and Exercise: Their Effects on Malondialdehyde and Lipid Peroxidation. Melatonin - Molecular Biology, Clinical and Pharmaceutical Approaches. 2018;
Takeshima T, Usui K, Mori K, Asai T, Yasuda K, Kuroda S, et al. Oxidative stress and male infertility. Reprod Med Biol. 2021;20(1):41–52.
Mohideen K, Sudhakar U, Balakrishnan T, Almasri MA, Al-Ahmari MM, Dira HS Al, et al. Malondialdehyde, an oxidative stress marker in oral squamous cell carcinoma—A systematic review and meta-analysis. Curr Issues Mol Biol. 2021;43(2):1019–35.
Jimenez-Lopez C, Carpena M, Lourenço-Lopes C, Gallardo-Gomez M, M. Lorenzo J, Barba FJ, et al. Bioactive compounds and quality of extra virgin olive oil. Foods. 2020;9(8).
Oliveras-López MJ, Berná G, Jurado-Ruiz E, López-García de la Serrana H, Martín F. Consumption of extra-virgin olive oil rich in phenolic compounds has beneficial antioxidant effects in healthy human adults. J Funct Foods [Internet]. 2014;10:475–84. Available from: http://dx.doi.org/10.1016/j.jff.2014.07.013
Laily EI, Saragih NP, Sirait LL. Pengaruh Penggunaan Extra Virgin Olive Oil Pencegahan Luka Tekan Pada Pasien Berisiko Dengan Skala Braden. Community of Publishing in Nursing (COPING). 2019;7(3):153–8.
Rahmawati W, Retnaningrum DN, Rahasti A. Pengaruh EVOO (Extra Virgin Olive Oil) Terhadap Tekanan Darah dan Kadar Protein Urin Pada Tikus Model Preeklamsia. Dinamika Kesehatan Jurnal Kebidanan dan Keperawatan. 2020;11(2):584–9.
Setiyono S, Gunardi S. Extra Virgin Olive Oil Dapat Menurunkan Kolesterol Total Pada Lansia Anggota Posbindu Kenanga. Jurnal Ilmiah Ilmu Keperawatan Indonesia. 2019;8(02):401–6.
Cicerale S, Lucas L, Keast R. Biological activities of phenolic compounds present in virgin olive oil. Int J Mol Sci. 2010;11(2):458–79.
Ghanbari R, Anwar F, Alkharfy KM, Gilani AH, Saari N. Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.)-A review. Int J Mol Sci. 2012;13(3):3291–340.
Astawan M, Wresdiyati T, Nasution NA. Fakta dan Manfaat Minyak Zaitun. Jakarta: Kompas Media Nusantara; 2015. 137 p.
Sánchez-Fidalgo S, Sánchez de Ibargüen L, Cárdeno A, Alarcón de la Lastra C. Influence of extra virgin olive oil diet enriched with hydroxytyrosol in a chronic DSS colitis model. Eur J Nutr [Internet]. 2012;51(4):497–506. Available from: https://doi.org/10.1007/s00394-011-0235-y
Akbaraly TN, Brunner EJ, Ferrie JE, Marmot MG, Kivimaki M, Singh-Manoux A. Dietary pattern and depressive symptoms in middle age. British Journal of Psychiatry. 2009;195(5):408–13.
Badiu D, Rajendram R. Effect of olive oil on the skin. In: Preedy VR, Watson RRBTO and OO in H and DP (Second E, editors. Olives and Olive Oil in Health and Disease Prevention [Internet]. 2nd ed. San Diego: Academic Press; 2021. p. 401–13. Available from: https://www.sciencedirect.com/science/article/pii/B9780128195284000328
Meilina. Extra virgin olive oil menurunkan kadar mda (Malondialdehyde) pada tikus (Rattus norvegicus) jantan galur wistar yang dipapar asap rokok. Intisari Sains Medis. 2017;8(2):97–101.
Hrapkiewicz K, Colby L, Denison P. Clinical Laboratory Animal Medicine. 4th ed. Lowa, USA: Wiley Blackwell; 2013. 449 p.
Hau J, Schapiro SJ. Handbook of Laboratory Animal Science Volume II - Animal Model. 3rd ed. Boca Raton, Florida, USA: CRC Press; 2011. 465 p.
Notoatmodjo S. Metodologi Penelitian Kesehatan. 3rd ed. Jakarta: Rineka Cipta; 2022. 268 p.
Federer WT. Randomization and Sample Size In Experimentation. Cornell University Biometrics Unit Technical [Internet]. 1966 Sep [cited 2024 Feb 7];Number BU-236-M:1–15. Available from: https://hdl.handle.net/1813/32334
NRC N. Guide for the Care and Use of Laboratory Animals. 8th ed. Vol. 39, The Physiologist. Washington, D.C.: National Academy Press; 2011. 246 p.
Lee SI, Kim JW, Lee YK, Yang SH, Lee IA, Suh JW, et al. Anti-obesity Effect of Monascus pilosus Mycelial Extract in High Fat Diet-induced Obese Rats. J Appl Biol Chem. 2011;54(3):197–205.
Roenigk HH, Auerbach R, Maibach H, Weinstein G, Lebwohl M. Methotrexate and psoriasis: Consensus conference. J Am Acad Dermatol. 1998;38(3):478–85.
Ghozali I. Aplikasi Analisis Multivariate dengan Program IBM SPSS 25. Badan Penerbit Universitas Diponegoro. Semarang; 2018.
Ghozali I. Aplikasi analisis multivariete SPSS 25. Semarang: Universitas Diponegoro; 2018.
Alsawaf S, Alnuaimi F, Afzal S, Thomas RM, Chelakkot AL, Ramadan WS, et al. Plant Flavonoids on Oxidative Stress-Mediated Kidney Inflammation. Biology 2022, Vol 11, Page 1717. 2022 Nov;11(12):1717.
Engwa GA. Free Radicals and the Role of Plant Phytochemicals as Antioxidants Against Oxidative Stress-Related Diseases. In: Phytochemicals - Source of Antioxidants and Role in Disease Prevention. IntechOpen; 2018. p. 49–73.
Christi WW, Harwoo JL. Oxidation of polyunsaturated fatty acids to produce lipid mediators. Essays Biochem. 2020;64(3):401–21.
Jialal I, Devaraj S. Low-density lipoprotein oxidation, antioxidants, and atherosclerosis: A clinical biochemistry perspective. Clin Chem. 1996;42(4):498–506.
Berumen J, Baglieri J, Kisseleva T, Mekeel K. Liver fibrosis: Pathophysiology and clinical implications. WIREs Mechanisms of Disease. 2021;13(1):1–25.
Bedossa P. Pathology of non-alcoholic fatty liver disease. Liver International. 2017;37(October 2016):85–9.
Winarti C, Innawati, Hemani. Physical Characteristics and UV Protection (In Vitro) of Gambier Leaf Extract Lotion. IOP Conf Ser Earth Environ Sci. 2022;1024(1).
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