Pengaruh Suhu Penyimpanan Terhadap Jumlah Eritrosit Pada Transfusi Darah di Rumah Sakit Bank Darah RSUD Dr. Soedarso Pontianak
Keywords:
Jumlah Eritrosit, Darah Transfusi, Automatic Cell Counter, Suhu PenyimpananAbstract
Transfusi adalah proses pemindahan darah dari donatur kepada resipien, guna memperbaiki kondisi anemia dengan menggunakan darah yang berkualitas baik. Darah sebelum didonasikan akan disimpan pada refrigerator. Pada penyimpanan, darah akan mengalami berbagai perubahan komponen, termasuk jumlah trombosit, jumlah eritrosit dan kadar hemoglobin. Penyimpanan PRC didalam blood bank dengan suhu standar 10C - 60C mengurangi lisis, pendinginan diharapkan memperlambat metabolisme, mengurangi metabolisme glukosa, meningkatkan kelangsungan hidup PRC. Penyimpanan RBC dengan pendinginan cepat di bawah 150C dapat mencegah hilangnya diphosphoglycerate (DPG) dari RBC. Penelitian ini bertujuan untuk mengetahui pengaruh suhu 20C, 40C, dan 60C terhadap jumlah eritrosit pada darah tranfusi di Bank Darah Rumah Sakit Umum Daerah Dr. Soedarso Pontianak. Desain penelitian berbentuk eksperimen semu. Teknik sampling yang digunakan adalah purposive sampling, dengan jumlah sampel sebanyak 27 sampel, dilakukan dengan 3 perlakuan, 9 kali pengulangan dengan suhu 20C, 40C dan 60C. Perhitungan eritrosit menggunakan metode Automatic Cell Counter. Hasil uji statistic Kendall’s tau didapatkan nilai p (0,673) < α 0,05 berarti Ha ditolak. Sehingga, tidak ada pengaruh suhu penyimpanan suhu 20C, 40C dan 60C terhadap jumlah eritrosit pada darah donor sukarela di Bank Darah Rumah Sakit Umum Daerah Dr. Soedarso Pontianak
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