Posttest Only Evaluation of Garlic Extract as Aedes aegypti Larvicide
Keywords:
Aedes aegypti, Allium sativum, botanical larvicide, dengue vector, larvae mortalityAbstract
Background: Dengue remains an important Aedes-borne disease, and Aedes aegypti is the primary vector in many endemic settings. The expansion of dengue risk, increasing insecticide resistance, and the need for environmentally acceptable vector-control options support the evaluation of plant-based larvicides. This study assessed the mortality of Aedes aegypti larvae exposed to garlic (Allium sativum) extract.
Methods: A true laboratory experiment with a posttest-only control group design was conducted at the Entomology Laboratory, Department of Environmental Health, Poltekkes Kemenkes Palu, in September 2023. Third-instar Aedes aegypti larvae were allocated to six groups: control (0 ppm) and garlic extract concentrations of 0.8, 0.9, 1.0, 1.1, and 1.2 ppm. Each group had four replications with 20 larvae per container (n = 480). Mortality was observed after 2, 4, 6, 12, and 24 hours and analyzed descriptively.
Results: No mortality occurred in the control group. Cumulative 24-hour mortality increased from 8/80 larvae (10.0%) at 0.8 ppm to 12/80 (15.0%) at 0.9 ppm, 13/80 (16.25%) at 1.0 ppm, 14/80 (17.5%) at 1.1 ppm, and 16/80 (20.0%) at 1.2 ppm.
Conclusion: Garlic extract produced a concentration-related increase in Aedes aegypti larval mortality, but concentrations below 2 ppm did not produce immediate high-level larval control within 24 hours. These low-concentration findings support further studies on sublethal effects, including delayed larval development, impaired pupation, and reduced adult emergence, alongside broader concentration ranges, lethal concentration analyses, and standard positive-control comparisons
References
Achee, N. L., Grieco, J. P., Vatandoost, H., Seixas, G., Pinto, J., Ching-Ng, L., Martins, A. J., Juntarajumnong, W., Corbel, V., Gouagna, C., David, J.-P., Logan, J. G., Orsborne, J., Marois, E., Devine, G. J., & Vontas, J. (2019). Alternative strategies for mosquito-borne arbovirus control. PLoS Neglected Tropical Diseases, 13(1), e0006822. https://doi.org/10.1371/journal.pntd.0006822
Agesti, E. F., Wardani, D. P. K., Mujahid, I., & Hikmawati, I. (2025). Efektivitas Ekstrak Bawang Putih (Allium sativum) Terhadap Mortalitas Larva Aedes aegypti: The Effectiveness of Garlic Extract (Allium sativum) Against the Mortality of Larvae Aedes aegypti. ASPIRATOR - Journal of Vector-Borne Diseases Studies, 16(2), 10–19. https://doi.org/10.58623/aspirator.v16i2.79
Assemie, A., & Gemeda, T. (2023). Larvicidal Activities of Allium sativum L. and Zingiber officinale Rosc. Extracts against Filariasis Vectors in Hadiya Zone, Ethiopia. BioMed Research International, 2023, 6636837. https://doi.org/10.1155/2023/6636837
Bharathithasan, M., Kotra, V., Abbas, S. A., & Mathews, A. (2024). Review on biologically active natural insecticides from Malaysian tropical plants against Aedes aegypti and Aedes albopictus. Arabian Journal of Chemistry, 17. https://doi.org/10.1016/j.arabjc.2023.105345
Dusfour, I., Vontas, J., David, J.-P., Weetman, D., Fonseca, D. M., Corbel, V., Raghavendra, K., Coulibaly, M. B., Martins, A. J., Kasai, S., & Chandre, F. (2019). Management of insecticide resistance in the major Aedes vectors of arboviruses: Advances and challenges. PLOS Neglected Tropical Diseases, 13(10), e0007615. https://doi.org/10.1371/journal.pntd.0007615
El-Saber Batiha, G., Magdy Beshbishy, A., G Wasef, L., Elewa, Y. H. A., A Al-Sagan, A., Abd El-Hack, M. E., Taha, A. E., M Abd-Elhakim, Y., & Prasad Devkota, H. (2020). Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review. Nutrients, 12(3), 872. https://doi.org/10.3390/nu12030872
Gan, S. J., Leong, Y. Q., bin Barhanuddin, M. F. H., Wong, S. T., Wong, S. F., Mak, J. W., & Ahmad, R. B. (2021). Dengue fever and insecticide resistance in Aedes mosquitoes in Southeast Asia: A review. Parasites & Vectors, 14(1), 315. https://doi.org/10.1186/s13071-021-04785-4
Hamid, P. H., Ninditya, V. I., Prastowo, J., Haryanto, A., Taubert, A., & Hermosilla, C. (2018). Current Status of Aedes aegypti Insecticide Resistance Development from Banjarmasin, Kalimantan, Indonesia. BioMed Research International, 2018, 1735358. https://doi.org/10.1155/2018/1735358
Kasman, K., Ishak, H., Alam, G., Amiruddin, R., Hastutiek, P., Arsin, A. A., Nasir, S., Ridha, M. R., & Wahid, I. (2025). Resistance status of Aedes mosquitoes as dengue vectors and the potential of plant larvicides from Indonesia for biological control: A narrative review. Narra J, 5(1), e1819–e1819. https://doi.org/10.52225/narra.v5i1.1819
Kementerian Kesehatan Republik Indonesia. (2024, November 14). Waspada Penyakit di Musim Hujan. Kementerian Kesehatan Republik Indonesia. https://kemkes.go.id/id/waspada-penyakit-di-musim-hujan
Kraemer, M. U. G., Reiner, R. C., Brady, O. J., Messina, J. P., Gilbert, M., Pigott, D. M., Yi, D., Johnson, K., Earl, L., Marczak, L. B., Shirude, S., Davis Weaver, N., Bisanzio, D., Perkins, T. A., Lai, S., Lu, X., Jones, P., Coelho, G. E., Carvalho, R. G., … Golding, N. (2019). Past and future spread of the arbovirus vectors Aedes aegypti and Aedes albopictus. Nature Microbiology, 4(5), 854–863. https://doi.org/10.1038/s41564-019-0376-y
Messina, J. P., Brady, O. J., Golding, N., Kraemer, M. U. G., Wint, G. R. W., Ray, S. E., Pigott, D. M., Shearer, F. M., Johnson, K., Earl, L., Marczak, L. B., Shirude, S., Davis Weaver, N., Gilbert, M., Velayudhan, R., Jones, P., Jaenisch, T., Scott, T. W., Reiner, R. C., & Hay, S. I. (2019). The current and future global distribution and population at risk of dengue. Nature Microbiology, 4(9), 1508–1515. https://doi.org/10.1038/s41564-019-0476-8
Mulyono, F. A., Romaidha, I., Khasanah, N. A. H., & Ngazizah, F. N. (2021). Efektivitas Ekstrak Bawang Putih (Allium sativum L.) Dengan Daun Bajakah (Uncaria acida Roxb.) Sebagai Larvasida Vektor Dengue Aedes aegypti. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 2(2), 152–164. https://doi.org/10.53699/joimedlabs.v2i2.50
Nasir, S., Walters, K. F. A., Pereira, R. M., Waris, M., Ali Chatha, A., Hayat, M., & Batool, M. (2022). Larvicidal activity of acetone extract and green synthesized silver nanoparticles from Allium sativum L. (Amaryllidaceae) against the dengue vector Aedes aegypti L. (Diptera: Culicidae). Journal of Asia-Pacific Entomology, 25(3), 101937. https://doi.org/10.1016/j.aspen.2022.101937
Nawarathne, M. P., & Dharmarathne, C. (2024). Control of dengue larvae of Aedes aegypti and Aedes albopictus using the larvicidal bioactive compounds in different plant extracts and plant extract-mediated nanoparticles. Tropical Medicine and Health, 52(1), 95. https://doi.org/10.1186/s41182-024-00654-9
Putra, A. I., & Delfita, R. (2021). Phytochemical Screening and Larvicidal Activity of Fermented Garlic to Aedes aegypti and Aedes albopictus (Diptera: Culicidae). Sainstek : Jurnal Sains dan Teknologi, 13(1), 47–57. https://doi.org/10.31958/js.v13i1.3236
Ryan, S. J., Carlson, C. J., Mordecai, E. A., & Johnson, L. R. (2019). Global expansion and redistribution of Aedes-borne virus transmission risk with climate change. PLOS Neglected Tropical Diseases, 13(3), e0007213. https://doi.org/10.1371/journal.pntd.0007213
Shang, A., Cao, S.-Y., Xu, X.-Y., Gan, R.-Y., Tang, G.-Y., Corke, H., Mavumengwana, V., & Li, H.-B. (2019). Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.). Foods, 8(7), 246. https://doi.org/10.3390/foods8070246
Silalahi, C. N., Tu, W.-C., Chang, N.-T., Singham, G. V., Ahmad, I., & Neoh, K.-B. (2022). Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. PLoS Neglected Tropical Diseases, 16(6), e0010501. https://doi.org/10.1371/journal.pntd.0010501
Tantowijoyo, W., Tanamas, S. K., Nurhayati, I., Setyawan, S., Budiwati, N., Fitriana, I., Ernesia, I., Wardana, D. S., Supriyati, E., Arguni, E., Meitika, Y., Prabowo, E., Andari, B., Green, B. R., Hodgson, L., Rancès, E., Ryan, P. A., O’Neill, S. L., Anders, K. L., … Simmons, C. P. (2022). Aedes aegypti abundance and insecticide resistance profiles in the Applying Wolbachia to Eliminate Dengue trial. PLoS Neglected Tropical Diseases, 16(4), e0010284. https://doi.org/10.1371/journal.pntd.0010284
Vasantha-Srinivasan, P., Srinivasan, K., Radhakrishnan, N., Han, Y. S., Karthi, S., Senthil-Nathan, S., Chellappandian, M., Babu, P., Ganesan, R., & Park, K. B. (2024). Larvicidal and enzyme inhibition effects of Phoenix pusilla derived Methyl oleate and malathion on Aedes aegypti strains. Scientific Reports, 14(1), 29327. https://doi.org/10.1038/s41598-024-79988-7
Wilder-Smith, A., Ooi, E.-E., Horstick, O., & Wills, B. (2019). Dengue. Lancet, 393(10169), 350–363. https://doi.org/10.1016/S0140-6736(18)32560-1
World Health Organization. (2023). Framework for national surveillance and control plans for Aedes vectors in the Pacific. World Health Organization. https://www.who.int/publications/i/item/9789290619758
World Health Organization. (2025). Dengue and severe dengue. World Health Organization. https://www.who.int/health-topics/dengue-and-severe-dengue
Yearsi, S. E. N., & Munawaroh, S. D. (2021). Effectiveness of Garlic Extract (Allium sativum L) as Larvicide of Aedes aegypti. Insights in Public Health Journal, 2(1). https://doi.org/10.20884/1.iphj.2021.2.1.4897
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